Did Lucifer’s sin in Heaven defile the Heavenly Sanctuary?
The biblical answer is NO.-"Nothing defiled shall enter the Kingdom of God."-Revelation21:27
22And I saw no temple therein. For the Lord God Almighty is the temple thereof, and the Lamb. 23And the city hath no need of the sun, nor of the moon, to shine in it. For the glory of God hath enlightened it, and the Lamb is the lamp thereof. 24And the nations shall walk in the light of it: and the kings of the earth shall bring their glory and honour into it. 25And the gates thereof shall not be shut by day: for there shall be no night there. 26And they shall bring the glory and honour of the nations into it. 27There shall not enter into it any thing defiled, or that worketh abomination or maketh a lie, but they that are written in the book of life of the Lamb. -Revelation21:22-27
There is no atonement for the wickedness and rebellion of Lucifer's and the fallen angels, because of the superb intellect and full-knowledge of their action. Perhaps, what you are wondering is, what is the real cause of Lucifer's fall, and in this mystery the revelation of Mary of Agreda can help you ponder and weave into it the passages in Exekiel chapter 28 and the book of Isaiah where Lucifer uttered the "I Will", or the desires of his heart that he thinks, God deprived him of, why?
To understand this mystery, the keyword is "Christ" or the "Anointed One". And what is the instrument of God's anointing? The instrument is seated in the Throne of God in the Holy Mountain, that Lucifer saw, and it was majestically beautiful. Lucifer, thinks, that if God "anoints" him again with this "Spirit that was seated at the Throne"-Wisdom9:1-ff, then he will become like unto God. Can you ponder this profound mystery?
In Ezekiel chapter 28, Lucifer received the "First Anointing", and since he was only a cherub, a little lower than Seraph, he cannot pass them, but the anointing of God, is the first source of Lucifer's delight, why? Lucifer was able to pass the fiery regions or the seraphic angels that is nearest to the Throne of God. Lucifer anointing gave him the cloth of superiority over the Seraphic Angels, and all the angels were in awe with Lucifer.
On the day that you were created
They were prepared.
14**“You were the anointed cherub who [h]covers**,
And I placed you there.
You were on the holy mountain of God;
You walked in the midst of the stones of fire.
15“You were blameless in your ways
From the day you were created
Until unrighteousness was found in you.
16“By the abundance of your trade
[i]You were internally filled with violence,
And you sinned;
Therefore I have cast you as profane
From the mountain of God.
And I have destroyed you, you [j]covering cherub,
From the midst of the stones of fire.
We can see that Lucifer was a very beautiful cherub wearing a priestly vestment or a celestial priest because he "bear the light of God" which is Jesus Christ the Eternal High Priest. But the beauty that God gave him, is not enough for him to surpass the "stones of fire or the fiery regions" that pointed to the Seraph but the "anointing of God" gave him extra powers to surpass the Seraph, as he now became the angel nearest to the Throne of God, which after he fall, was replaced by Archangel Gabriel. And this privilege, allowed him to saw the "Throne of God" and most esp, the "Spirit of Created Wisdom" (Proverbs8:22), that was seated on the Throne. Lucifer thought that God will anoint him again with the "Spirit of Wisdom", and this is the start of Lucifer's desire that leads to his wickedness and eventually rebellion, because he realize as seen by Mary of Agreda visions, that when God revealed to the angelic host His Divine Plan, the "Spirit of Wisdom" that was seated on the Throne, belongs to Jesus the "Anointed One", and ultimately this "Spirit of Wisdom" will rest on a Tabernacle, the greatest masterpiece creation of God, called the "Immaculate Heart of Mary the Tabernacle of God".
 And by my power I have trodden under my feet the hearts of all the high and low: and in all these I sought rest, and I shall abide in the inheritance of the Lord.  Then the creator of all things commanded, and said to me: and he that made me, rested in my tabernacle,  And he said to me: Let thy dwelling be in Jacob, and thy inheritance in Israel, and take root in my elect.  From the beginning, and before the world, was I created, and unto the world to come I shall not cease to be, and in the holy dwelling place I have ministered before him.  And so was I established in Sion, and in the holy city likewise I rested, and my power was in Jerusalem.- Sirach24:11-15
Lucifer's heart or desire was expose by Ezekiel 28, he desires to seat in the Throne of God. And the only way to sit on the throne is for Lucifer to receive the anointing of the "Spirit of Wisdom" that was seated at the Throne. But the Spirit of Wisdom is not birthed or created for the angels, it was destined for Jesus as the "Anointed One" and ultimately destined for the "Immaculate Heart" of the chosen Woman, who will give the "Spirit of Wisdom" a perfect dwelling place or a throne or a tabernacle as described in Sirach24:12 and Proverbs9:1.
How then Lucifer convince other angels to rebel?
Because, Lucifer gave them a promise that if God will anoint him with the Spirit of Wisdom, he will assigned them to different position, Lucifer began to trade, because he claimed that, he will not only infuse them with "light that he bears" he will also "give them gifts of wisdom". The third of the angels also desires to have an advantage with other angels by possessing the gift of wisdom. And Lucifer convinced the third of the angels to take side with him. But all this "trade" shattered, when God revealed His Divine Plan. Lucifer and the third of the angels was shocked, because the "Anointed One" was revealed, and its not Lucifer but Jesus the Christ, the Anointed One of God. Lucifer desires of experiencing to be adore by all the angels in Heaven, must now adore a God-Man, Jesus Christ. This was tolerable to the third of the angels, but the thing that make them rebel was, when God revealed, that the Spirit of Wisdom will ultimately rest upon the chosen Woman, and so, Lucifer and all the angels must also bow down to the chosen Woman because She was destined to sit on the Throne of God on the Holy Mountain.
The Spirit of Wisdom descended on Jesus Baptism at Jordan River and He became the "Anointed One", the First Advocate.
The Spirit of Wisdom, return to the Abba Father, and the ascended Jesus first act of mediation to the Abba Father was to send the Spirit of Wisdom at the Upper Room, and in the Upper Room the Spirit of Wisdom finally dwell and rested in the Immaculate Heart of Mary the Tabernacle of God and She became the *"another Advocate"*as St.Alphosnsus Liguori a Doctor of the Church teaches, by pointing to Mary as the another Advocate. The Immaculate Heart of Mary was hewn by the Seven Pillars or the Seven Sorrows in order for the Spirit of Wisdom to have a perfect dwelling place.-Proverbs9:1
Wisdom has built her house; she has carved out her seven pillars.
Mary Seat of Wisdom, pray for us. S&IHMMP4us. Amen
More on this story on the revelation of Mary of Agreda titled "Mystical City of God.