Biblical basis for claiming Eve was a virgin prior to the Fall
In other words did Adam and Eve have sex with each other in the Garden of Eden?
Sure, it is possible, since the Scriptures do not say clearly one way or another. However according to tradition, the Church Fathers do not think so. If Adam and Eve did have intimacy of a sexual nature before the fall, there would have been, to what degree I do not know, a chance that Eve could have conceived a child and that child would have been born without original sin! This fact alone must be weighed in on the minds of all the Church Fathers in this issue. Even St. Paul elaborated that sin entered the world through one man. Why would God allow the act of sexual intercourse while in the Garden to alter the outcome of Original Sin.
From reading Scriptures alone it is impossible to say one way or another, if Eve could have known Adam intimately.
The Bible does not say whether or not Adam and Eve had sexual relations in the Garden of Eden. Therefore, we cannot say they did or did not. Still, let's take a look at the issue a little more deeply.
"Then God said, “Let Us make man in Our image, according to Our likeness; and let them rule over the fish of the sea and over the birds of the sky and over the cattle and over all the earth, and over every creeping thing that creeps on the earth. 27 God created man in His own image, in the image of God He created him; male and female He created them. 28 God blessed them; and God said to them, “Be fruitful and multiply, and fill the earth, and subdue it; and rule over the fish of the sea and over the birds of the sky and over every living thing that moves on the earth," (Genesis 1:26-25).
God commanded them to multiply and fill the earth. But, we do not know if they had begun to fulfill that command by having relations in the Garden. Furthermore, we do not know how long they were in the Garden before they fell.
In Genesis 2 there is a more detailed account of the creation order that Genesis 1:26-25 goes over.
Man is formed. "Then the LORD God formed man of dust from the ground, and breathed into his nostrils the breath of life; and man became a living being," (Genesis 2:7).
Not good for man to be alone. "Then the LORD God said, “It is not good for the man to be alone; I will make him a helper suitable for him," (Genesis 2:18). God made Eve. "So the LORD God caused a deep sleep to fall upon the man, and he slept; then He took one of his ribs and closed up the flesh at that place, 22 the LORD God fashioned into a woman the rib which He had taken from the man, and brought her to the man." (Genesis 2:21-22).
So, there is no way to know for sure whether or not Adam and Eve actually had sexual relations before they were expelled from the Garden of Eden. - Did Adam and Eve have sex with each other in the Garden of Eden?
Thus we are forced to take a look at one more sacred text before moving on.
When God put Adam and Eve out of the Garden, He told Eve the following:
I will greatly multiply thy sorrow and thy conception; in sorrow thou shalt bring forth children; and thy desire shall be to thy husband, and he shall rule over thee. - Genesis 3:16
This last text tends to imply that both Adam and Eve did not have sexual relations while in the Garden, for now they knew that such relationship could result in a painful delivery of a child for all women. God had foreseen this moment to reveal this punishment for sinning in the Garden.
Again tradition holds that both Adam and Eve were virgins in the Garden. St. Justin Martyr, writing about 155 A.D. in his work 100 Dialogue with Trypho, and St. Irenaeus, writing about 189 A.D. in his work 3, 22 Against Heresies, both say Eve was still a virgin at the time she and Adam committed the first sin.
Immediately after eating the forbidden fruit, both Adam and Eve knew they were naked. This particular text begs the question of whether they simply knew they were naked or whether they may have been previously dressed by God while in conformity with his will.
6So when the woman saw that the tree was good for food, that it was pleasant to the eyes, and a tree desirable to make one wise, she took of its fruit and ate. She also gave to her husband with her, and he ate. 7Then the eyes of both of them were opened, and they knew that they were naked; and they sewed fig leaves together and made themselves coverings. - Genesis 3: 6-7
Several early interpreters speak of Adam and Even being stripped of their clothing. For example, Ephraem (4th century Syrian theologian) says:
It is because of the glory with which they were clothed that they were not ashamed. When it was taken away from them after they had violated the commandment.They were indeed ashamed, because they were now naked (Commentary on Genesis, 2:14). - Genesis, Creation, and Ancient Interpreters: In the Garden
St. John Chrysostom as well seems to support this idea:
[W]hile sin and disobedience had not yet come on the scene, they were clad in that glory from above which caused them no shame. But after the breaking of the law, then entered the scene both shame and awareness of their nakedness. (Louth, Conti and Oden, Ancient Christian Commentary on Scripture: Old Testament I, Genesis 1-11 2001, 72)
One thus asks the question that if Eve and Adam were actually clothed in light would they have been intimate with one another while at the same time being so intimately united to their creator and clothed in God’s light of glory.
According to Scriptures, it is only after they sinned that Adam knew his wife. There is no reason not to accept that that Genesis is not chronological accurate.
4 And Adam knew Eve his wife; and she conceived, and bare Cain, and said, I have gotten a man from the Lord. - Genesis 4:1
The tradition that both Adam and Eve were clothed in light is followed in some Jewish thoughts (Clothing of Light) and is revealed in the revelations of Blessed Catherine Emmerich.
Even if one does not believe that Eve was a virgin in the Garden, tradition and the Church Fathers hold that she was virgin as well as Adam.